Complete sequence of VP2 gene of the bluetongue virus serotype 1 (BTV-1).
نویسندگان
چکیده
A clone representing a full length cDNA copy of the bluetongue virus (BTV) outer capsid protein VP2 gene (serotype 1, isolated in Australia) was obtained using methods described elsewhere (1). The complete sequence of the gene determined by dideoxy method (2) was deduced to be 2940 base pairs long. It codes for a primary gene product of 962 amino acids with an estimated M.W. 112,087, a comparable size to the VP2 proteins of US BTV serotypes (1,3).
منابع مشابه
Variation in the bluetongue virus neutralization protein VP2.
To determine the extent and nature of the antigenic variation of four U.S.A. serotypes of bluetongue virus (BTV), the complete nucleotide sequence was determined for cDNA clones representing the L2 dsRNA of BTV serotype 13, the gene that codes for the outer capsid neutralization antigen (VP2). The predicted amino acid sequence of the protein was compared with the VP2 sequences of the U.S.A. ser...
متن کاملAnalysis and phylogenetic comparisons of full-length VP2 genes of the 24 bluetongue virus serotypes.
The outer capsid protein VP2 of Bluetongue virus (BTV) is a target for the protective immune response generated by the mammalian host. VP2 contains the majority of epitopes that are recognized by neutralizing antibodies and is therefore also the primary determinant of BTV serotype. Full-length cDNA copies of genome segment 2 (Seg-2, which encodes VP2) from the reference strains of each of the 2...
متن کاملRestriction analysis of conserved and variable regions of VP2 gene of Indian isolates of bluetongue virus serotype 1.
Bluetongue virus (BTV) is a member of Orbivirus genus in family Reoviridae. The virus genome is composed of 10 double-stranded RNA segments. The RNA segment L2 encodes an outer capsid viral protein VP2, which is the main determinant of neutralization and serotype-specific immune response. BTV serotype 1 (BTV-1) specific novel primer pair was designed using VP2 gene sequences available in GenBan...
متن کاملSegment 2 based characterization of a novel Indian bluetongue virus isolate
Aim: The study was conducted to characterize and serotype the novel isolate of bluetongue virus (BTV) isolated from India. Materials and Methods: The BTV isolate was propagated in BHK-21 cell line. Nucleic acid (dsRNA) was extracted using Trizol method and cDNA was prepared using a process called reverse transcription. The cDNA was subjected to group specific PCR using ns1 gene specific primer ...
متن کاملIsolation of bluetongue virus serotype-1 from aborted goat foetuses
Abortions and stillbirths were noticed in pregnant goats on a farm in the state of Gujarat, India. About 50% of the pregnant goats aborted or gave birth to dead kids. Bluetongue virus (BTV) antibody in the sera of affected goats was detected using a competitive enzyme-linked immunosorbent assay (ELISA). Viral antigen in the blood of these goats and in the aborted fetal spleens was detected usin...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Nucleic acids research
دوره 16 6 شماره
صفحات -
تاریخ انتشار 1988